|
|
|
| Registros recuperados: 35 | |
|
|
| Balbuena,Tiago S.; Dias,Leonardo L. C.; Martins,Mariana L. B.; Chiquieri,Tatiana B.; Santa-Catarina,Claudete; Floh,Eny I. S.; Silveira,Vanildo. |
| Genome sequencing of various organisms allow global analysis of gene expression, providing numerous clues on the biological function and involvement in the biological processes studied. Proteomics is a branch of molecular biology and biotechnology that has undergone considerable development in the post-genomic era. Despite the recent significant advancements in proteomics techniques, still there is much to be improved. Due to peculiarities to the plant kingdom, proteomics approaches require adaptations, so as to improve efficiency and accuracy of results in plants. Data generated by proteomics can substantially contribute to the understanding and monitoring of plant physiological events and development of biotechnological strategies. Especially for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2-DE; Mass spectrometry; Recalcitrant species; Proteomics; Unknown genome. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000200001 |
| |
|
|
| Barranger, Audrey; Langan, Laura M.; Sharma, Vikram; Rance, Graham A.; Aminot, Yann; Weston, Nicola J.; Akcha, Farida; Moore, Michael N.; Arlt, Volker M.; Khlobystov, Andrei N.; Readman, James W.; Jha, Awadhesh N.. |
| This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by Gas Chromatography–Mass Spectrometry (GC–MS), Liquid Chromatography–High Resolution Mass Spectrometry (LC–HRMS) and Inductively Coupled Plasma Mass Spectrometer (ICP–MS). Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the interactive effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1... |
| Tipo: Text |
Palavras-chave: Trojan horse effect; B[a]P; NC(60); Co-exposure; Mussels; DNA damage; Proteomics. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00506/61780/65779.pdf |
| |
|
|
| Barranger, Audrey; Langan, Laura M; Sharma, Vikram; Rance, Graham A; Aminot, Yann; Weston, Nicola J; Akcha, Farida; Moore, Michael N; Arlt, Volker M; Kholbystov, Andrei N; Readman, James W; Jha, Awadhesh N.. |
| This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by GC-MS, LC-HRMS and ICP-MS. Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the toxic effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1 mg/L of C60, the majority of which were down-regulated (~52%). No DEPs were identified at any of the concentrations of nC60 (p < 0.05, 1% FDR).... |
| Tipo: Text |
Palavras-chave: Trojan Horse effect; B[a]P; NC60; Co-exposure; Mussels; DNA damage; Proteomics. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00498/60946/64340.pdf |
| |
|
| |
|
|
| Chetouhi, Cherif; Masseret, Estelle; Satta, Cecilia Teodora; Balliau, Thierry; Laabir, Mohamed; Jean, Natacha. |
| Over the past decades, the occurrence, distribution and intensity of harmful algal blooms involving the dinoflagellate Alexandrium pacificum have increased in marine coastal areas disturbed by anthropogenic inputs. This invasive species produces saxitoxin, which causes the paralytic shellfish poisoning syndrome in humans upon consumption of contaminated seafood. Blooms of A. pacificum have been reported in metal-contaminated coastal ecosystems, suggesting some ability of these microorganisms to adapt to and/or resist in metal stress conditions. This study seeks to characterize the modifications in membrane proteomes (by 2-D electrophoresis coupled to LC-MS/MS), cell growth and morphometry (measured with an inverted microscope), in response to metal stress... |
| Tipo: Text |
Palavras-chave: Alexandrium pacificum; Harmful algal bloom; Proteomics; Membrane pmteome; Trace metals. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71802/70290.pdf |
| |
|
| |
|
|
| Corporeau, Charlotte; Vanderplancke, Gwenaelle; Boulais, Myrina; Suquet, Marc; Quere, Claudie; Boudry, Pierre; Huvet, Arnaud; Madec, Stephanie. |
| We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially expressed between females showing high or low oocyte quality were identified by nano-liquid chromatography tandem mass spectrometry. Twelve up-accumulated spots associated with low quality oocytes revealed 10 distinct proteins, including vitellogenin — breakdown products and metabolic enzymes. Eight up-accumulated spots from high quality oocytes revealed 6 distinct proteins, including chaperone molecules and... |
| Tipo: Text |
Palavras-chave: Marine bivalves; Crassostrea gigas; Oocyte quality; Proteomics. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00098/20925/18534.pdf |
| |
|
|
| Cui,Lunbiao; Ge,Yiyue; Qi,Yuhua; Shi,Zhiyang; Jiao,Yongjun; Qi,Xian; Zhai,Xiangjun; Wang,Hua. |
| The PreS1 region of the L protein is important in cell attachment and consequently in hepatitis B virus (HBV) infectivity. To identify novel PreS1 interacting protein, we performed Glutathione-S-transferase (GST) pull-down, two-dimensional gel electrophoresis (2-DE) and mass spectrometry assays. Glucose-regulated proteins (GRP) 78 and 75 were found to bind PreS1. The interactions between PreS1 and GRP75 were confirmed by a co-immunoprecipitation experiment. GRP78 and GRP75 may play important roles in mediating HBV virion entering into hepatocyte and regulating proper folding of the L protein due to their critical functions in protein folding and trafficking. The finding of novel PreS1 binding protein enriches our knowledge about molecular mechanism of HBV... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HBV; PreS1; Proteomics; GRP75. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200036 |
| |
|
| |
|
| |
|
|
| Hamza, Neila; Silvestre, Frederic; Mhetli, Mohamed; Ben Khemis, Ines; Dieu, Marc; Raes, Martine; Cahu, Chantal; Kestemont, Patrick. |
| A comparative proteomic approach was used to assess the protein expression profile in the liver of 34 days old pikeperch larvae fed from day 10 post hatching, with three isoproteic and isolipidic formulated diets varying by their phospholipid (PL) contents (% dry diet weight): 1.4% (PL1), 4.7% (PL5) and 9.5% (PL9). Using 2D-DIGE minimal labelling of liver extracts, we were able to show 56 protein spots with a differential intensity (p<0.05) depending on the dietary PL content. Among these spots, 11 proteins were un-ambiguously identified using nanoLC-MS/MS tandem mass spectrometry. In the PL9 larvae, our results indicate that the glycolytic pathway could be down-regulated due to the under-expression of the fructose biphosphate aldolase B and the... |
| Tipo: Text |
Palavras-chave: 2D-DIGE; Metabolism; Phospholipid; Pikeperch; Proteomics. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00007/11837/8575.pdf |
| |
|
| |
|
|
| Jia,Yan-Long; Chen,Hui; Zhang,Chong; Gao,Li-Jie; Wang,Xi-Cheng; Qiu,Le-Le; Wu,Jun-Fang. |
| Abstract Dunaliella salina, a single-celled marine alga with extreme salt tolerance, is an important model organism for studying fundamental extremophile survival mechanisms and their potential practical applications. In this study, two-dimensional differential in-gel electrophoresis (2D-DIGE) was used to investigate the expression of halotolerant proteins under high (3 M NaCl) and low (0.75 M NaCl) salt concentrations. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) and bioinformatics were used to identify and characterize the differences among proteins. 2D-DIGE analysis revealed 141 protein spots that were significantly differentially expressed between the two salinities. Twenty-four differentially... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dunaliella salina; Halotolerant protein; Proteomics; Two-dimensional differential in-gel electrophoresis. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200239 |
| |
|
| |
|
| |
|
| |
|
|
| Leprêtre, Maxime; Faury, Nicole; Segarra, Amelie; Claverol, Stéphane; Degremont, Lionel; Palos-ladeiro, Mélissa; Armengaud, Jean; Renault, Tristan; Morga, Benjamin. |
| Massive mortality outbreaks affecting Pacific oysters (Crassostrea gigas) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted Pacific oyster families for their susceptibility to viral infection. Live oysters were sampled at 12, 26, and 144 h post infection (hpi) to analyze host-pathogen interactions using comparative proteomics. Shotgun proteomics allowed the detection of seven viral proteins in infected oysters, some of them with potential immunomodulatoy functions. Viral proteins were mainly detected in susceptible oysters sampled at 26 hpi, which correlates with the mortality and... |
| Tipo: Text |
Palavras-chave: Proteomics; Interactions; Antiviral response; OsHV-1; Pacific oysters. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00669/78075/80307.pdf |
| |
|
|
| Lomonte,Bruno; Calvete,Juan J.. |
| Abstract This work offers a general overview on the evolving strategies for the proteomic analysis of snake venoms, and discusses how these may be combined through diverse experimental approaches with the goal of achieving a more comprehensive knowledge on the compositional, toxic, and immunological characteristics of venoms. Some recent developments in this field are summarized, highlighting how strategies have evolved from the mere cataloguing of venom components (proteomics/venomics), to a broader exploration of their immunological (antivenomics) and functional (toxicovenomics) characteristics. Altogether, the combination of these complementary strategies is helping to build a wider, more integrative view of the life-threatening protein cocktails... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venoms; Proteomics; Venomics; Antivenomics; Toxicovenomics. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100206 |
| |
|
|
| MADROÑERO,Leidy Johana; CORREDOR-ROZO,Zayda-Lorena; ESCOBAR-PÉREZ,Javier; VELANDIA-ROMERO,Myriam-Lucía. |
| ABSTRACT Crop production and trade are two of the most economically important activities in Colombia, and viral diseases cause a high negative impact to agricultural sector. Therefore, the detection, diagnosis, control, and management of viral diseases are crucial. Currently, Next-Generation Sequencing (NGS) and 'Omic' technologies constitute a right-hand tool for the discovery of novel viruses and for studying virus-plant interactions. This knowledge allows the development of new viral diagnostic methods and the discovery of key components of infectious processes, which could be used to generate plants resistant to viral infections. Globally, crop sciences are advancing in this direction. In this review, advancements in 'omic' technologies and their... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NGS; Plant-virus interactions; Plant virology; Proteomics; Viral genomics. |
| Ano: 2019 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300423 |
| |
|
|
| Mariano,Douglas Oscar Ceolin; Messias,Marcela Di Giacomo; Spencer,Patrick Jack; Pimenta,Daniel Carvalho. |
| ABSTRACT Background: Bufonid parotoid macrogland secretion contains several low molecular mass molecules, such as alkaloids and steroids. Nevertheless, its protein content is poorly understood. Herein, we applied a sample preparation methodology that allows the analysis of viscous matrices in order to examine its proteins. Methods: Duttaphrynus melanostictus parotoid macrogland secretion was submitted to ion-exchange batch sample preparation, yielding two fractions: salt-displaced fraction and acid-displaced fraction. Each sample was then fractionated by anionic-exchange chromatography, followed by in-solution proteomic analysis. Results: Forty-two proteins could be identified, such as acyl-CoA-binding protein, alcohol dehydrogenase, calmodulin,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amphibian skin secretion; Bufonidae; Duttaphrynus melanostictus; Proteomics; Batch chromatography; Asian common toad. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100318 |
| |
| Registros recuperados: 35 | |
|
|
|
